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a549 cells human lung cancer derived aecs  (ATCC)


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    ATCC a549 cells human lung cancer derived aecs
    FIGURE 1. IL-37 protein levels were decreased in the lungs of patients with IPF. (A) Immunohistochemical staining of IL-37 in human lungs. IL-37 expression levels were examined in normal subjects (NSs) and in IPF lungs. IL-37 was strongly localized in <t>AECs</t> (inset) and alveolar macrophages (red arrows) in NSs. Original magnification 340, inset; original magnification 31000. (B) Lung IL-37 expression was measured by immunoblotting. Densi- tometry of IL-37 bands. Equal amounts of proteins obtained from lung lysates were subjected to immunoblotting with anti–IL-37 Ab. The blots were stripped and reprobed with anti–b-actin Ab. Lung tissues were obtained from patients with IPF and NSs who underwent thoracic surgery. *p , 0.05 versus NS. BALF (C) and serum (D) levels of IL-37, measured by ELISA. Dashed line represents the detection limit. *p , 0.05 versus NS.
    A549 Cells Human Lung Cancer Derived Aecs, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 9091 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/a549+cells+human+lung+cancer+derived+aecs/A549/pm31519861-139-3-11
    Average 99 stars, based on 9091 article reviews
    a549 cells human lung cancer derived aecs - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "IL-37 Attenuates Lung Fibrosis by Inducing Autophagy and Regulating TGF-β1 Production in Mice."

    Article Title: IL-37 Attenuates Lung Fibrosis by Inducing Autophagy and Regulating TGF-β1 Production in Mice.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1801515

    FIGURE 1. IL-37 protein levels were decreased in the lungs of patients with IPF. (A) Immunohistochemical staining of IL-37 in human lungs. IL-37 expression levels were examined in normal subjects (NSs) and in IPF lungs. IL-37 was strongly localized in AECs (inset) and alveolar macrophages (red arrows) in NSs. Original magnification 340, inset; original magnification 31000. (B) Lung IL-37 expression was measured by immunoblotting. Densi- tometry of IL-37 bands. Equal amounts of proteins obtained from lung lysates were subjected to immunoblotting with anti–IL-37 Ab. The blots were stripped and reprobed with anti–b-actin Ab. Lung tissues were obtained from patients with IPF and NSs who underwent thoracic surgery. *p , 0.05 versus NS. BALF (C) and serum (D) levels of IL-37, measured by ELISA. Dashed line represents the detection limit. *p , 0.05 versus NS.
    Figure Legend Snippet: FIGURE 1. IL-37 protein levels were decreased in the lungs of patients with IPF. (A) Immunohistochemical staining of IL-37 in human lungs. IL-37 expression levels were examined in normal subjects (NSs) and in IPF lungs. IL-37 was strongly localized in AECs (inset) and alveolar macrophages (red arrows) in NSs. Original magnification 340, inset; original magnification 31000. (B) Lung IL-37 expression was measured by immunoblotting. Densi- tometry of IL-37 bands. Equal amounts of proteins obtained from lung lysates were subjected to immunoblotting with anti–IL-37 Ab. The blots were stripped and reprobed with anti–b-actin Ab. Lung tissues were obtained from patients with IPF and NSs who underwent thoracic surgery. *p , 0.05 versus NS. BALF (C) and serum (D) levels of IL-37, measured by ELISA. Dashed line represents the detection limit. *p , 0.05 versus NS.

    Techniques Used: Immunohistochemical staining, Staining, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

    FIGURE 2. IL-37 is expressed in the AECs and macrophages in the mouse lung and are decreased by BLM. (A) Immunohistochemical staining of IL-37 in mouse lungs. IL-37 was strongly localized in AECs (inset) and alveolar macrophages in the control lung. Original magnification 3100, inset, original magnifi- cation 3400. (B) IL-37 expression was quantified by immunoblotting and densitometric analysis (n = 6 per group). *p , 0.05 versus control. Equal amounts of proteins obtained from lung lysates were subjected to immunoblotting with anti–IL-37 Ab. The blots were stripped and reprobed with anti–b-actin Ab.
    Figure Legend Snippet: FIGURE 2. IL-37 is expressed in the AECs and macrophages in the mouse lung and are decreased by BLM. (A) Immunohistochemical staining of IL-37 in mouse lungs. IL-37 was strongly localized in AECs (inset) and alveolar macrophages in the control lung. Original magnification 3100, inset, original magnifi- cation 3400. (B) IL-37 expression was quantified by immunoblotting and densitometric analysis (n = 6 per group). *p , 0.05 versus control. Equal amounts of proteins obtained from lung lysates were subjected to immunoblotting with anti–IL-37 Ab. The blots were stripped and reprobed with anti–b-actin Ab.

    Techniques Used: Immunohistochemical staining, Staining, Control, Expressing, Western Blot

    FIGURE 3. Antiapoptotic effect of IL-37 on BLM-treated AECs. (A) Mouse primary AECs were exposed to BLM (10 mg/ml) and/or human IL-37 (10, 100, and 1000 ng/ml) for 24 h in serum-free medium. *p , 0.05 versus apoptosis or necrosis of the BLM+/IL-372–treated groups. Apoptosis was defined as Annexin V+/both PI+ and PI2. Necrosis was defined as PI+/both Annexin V+ and Annexin V2. (B) IL-37 siRNA and scrambled siRNA were transfected into A549 cells. BLM (10 mg/ml) was added 48 h post-siRNA transfection, and the samples were analyzed after 24 h. The cells were double stained with Annexin V/PI and analyzed by flow cytometry. *p , 0.05 versus apoptosis or necrosis of the scramble/BLM+-treated groups.
    Figure Legend Snippet: FIGURE 3. Antiapoptotic effect of IL-37 on BLM-treated AECs. (A) Mouse primary AECs were exposed to BLM (10 mg/ml) and/or human IL-37 (10, 100, and 1000 ng/ml) for 24 h in serum-free medium. *p , 0.05 versus apoptosis or necrosis of the BLM+/IL-372–treated groups. Apoptosis was defined as Annexin V+/both PI+ and PI2. Necrosis was defined as PI+/both Annexin V+ and Annexin V2. (B) IL-37 siRNA and scrambled siRNA were transfected into A549 cells. BLM (10 mg/ml) was added 48 h post-siRNA transfection, and the samples were analyzed after 24 h. The cells were double stained with Annexin V/PI and analyzed by flow cytometry. *p , 0.05 versus apoptosis or necrosis of the scramble/BLM+-treated groups.

    Techniques Used: Transfection, Staining, Cytometry

    Related Articles

    Knockdown:

    Article Title: IL-37 Attenuates Lung Fibrosis by Inducing Autophagy and Regulating TGF-β1 Production in Mice.
    Article Snippet: .. IL-37 knockdown in A549 cells Human lung cancer–derived AECs (A549 cells, ATCC CCL185; American Type Culture Collection, Manassas, VA) treated with or without BLM (10 mg/ml) were seeded on six-well plates and cultured in RPMI 1640 medium supplemented with 10% (v/v) FBS, 100 U/ml penicillin, and 100 mg/ml streptomycin. .. The cells were grown to 80% confluence and transfected with small interfering RNA (siRNA) using Lipofectamine RNAiMAX (Invitrogen, Life Technologies, Carlsbad, CA) in serum-free Opti-MEM.

    Cell Culture:

    Article Title: IL-37 Attenuates Lung Fibrosis by Inducing Autophagy and Regulating TGF-β1 Production in Mice.
    Article Snippet: .. IL-37 knockdown in A549 cells Human lung cancer–derived AECs (A549 cells, ATCC CCL185; American Type Culture Collection, Manassas, VA) treated with or without BLM (10 mg/ml) were seeded on six-well plates and cultured in RPMI 1640 medium supplemented with 10% (v/v) FBS, 100 U/ml penicillin, and 100 mg/ml streptomycin. .. The cells were grown to 80% confluence and transfected with small interfering RNA (siRNA) using Lipofectamine RNAiMAX (Invitrogen, Life Technologies, Carlsbad, CA) in serum-free Opti-MEM.



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    a549 cells human lung cancer derived aecs  (ATCC)
    99
    ATCC a549 cells human lung cancer derived aecs
    FIGURE 1. IL-37 protein levels were decreased in the lungs of patients with IPF. (A) Immunohistochemical staining of IL-37 in human lungs. IL-37 expression levels were examined in normal subjects (NSs) and in IPF lungs. IL-37 was strongly localized in <t>AECs</t> (inset) and alveolar macrophages (red arrows) in NSs. Original magnification 340, inset; original magnification 31000. (B) Lung IL-37 expression was measured by immunoblotting. Densi- tometry of IL-37 bands. Equal amounts of proteins obtained from lung lysates were subjected to immunoblotting with anti–IL-37 Ab. The blots were stripped and reprobed with anti–b-actin Ab. Lung tissues were obtained from patients with IPF and NSs who underwent thoracic surgery. *p , 0.05 versus NS. BALF (C) and serum (D) levels of IL-37, measured by ELISA. Dashed line represents the detection limit. *p , 0.05 versus NS.
    A549 Cells Human Lung Cancer Derived Aecs, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/a549+cells+human+lung+cancer+derived+aecs/A549/pm31519861-139-3-11
    Average 99 stars, based on 1 article reviews
    a549 cells human lung cancer derived aecs - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    FIGURE 1. IL-37 protein levels were decreased in the lungs of patients with IPF. (A) Immunohistochemical staining of IL-37 in human lungs. IL-37 expression levels were examined in normal subjects (NSs) and in IPF lungs. IL-37 was strongly localized in AECs (inset) and alveolar macrophages (red arrows) in NSs. Original magnification 340, inset; original magnification 31000. (B) Lung IL-37 expression was measured by immunoblotting. Densi- tometry of IL-37 bands. Equal amounts of proteins obtained from lung lysates were subjected to immunoblotting with anti–IL-37 Ab. The blots were stripped and reprobed with anti–b-actin Ab. Lung tissues were obtained from patients with IPF and NSs who underwent thoracic surgery. *p , 0.05 versus NS. BALF (C) and serum (D) levels of IL-37, measured by ELISA. Dashed line represents the detection limit. *p , 0.05 versus NS.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: IL-37 Attenuates Lung Fibrosis by Inducing Autophagy and Regulating TGF-β1 Production in Mice.

    doi: 10.4049/jimmunol.1801515

    Figure Lengend Snippet: FIGURE 1. IL-37 protein levels were decreased in the lungs of patients with IPF. (A) Immunohistochemical staining of IL-37 in human lungs. IL-37 expression levels were examined in normal subjects (NSs) and in IPF lungs. IL-37 was strongly localized in AECs (inset) and alveolar macrophages (red arrows) in NSs. Original magnification 340, inset; original magnification 31000. (B) Lung IL-37 expression was measured by immunoblotting. Densi- tometry of IL-37 bands. Equal amounts of proteins obtained from lung lysates were subjected to immunoblotting with anti–IL-37 Ab. The blots were stripped and reprobed with anti–b-actin Ab. Lung tissues were obtained from patients with IPF and NSs who underwent thoracic surgery. *p , 0.05 versus NS. BALF (C) and serum (D) levels of IL-37, measured by ELISA. Dashed line represents the detection limit. *p , 0.05 versus NS.

    Article Snippet: IL-37 knockdown in A549 cells Human lung cancer–derived AECs (A549 cells, ATCC CCL185; American Type Culture Collection, Manassas, VA) treated with or without BLM (10 mg/ml) were seeded on six-well plates and cultured in RPMI 1640 medium supplemented with 10% (v/v) FBS, 100 U/ml penicillin, and 100 mg/ml streptomycin.

    Techniques: Immunohistochemical staining, Staining, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

    FIGURE 2. IL-37 is expressed in the AECs and macrophages in the mouse lung and are decreased by BLM. (A) Immunohistochemical staining of IL-37 in mouse lungs. IL-37 was strongly localized in AECs (inset) and alveolar macrophages in the control lung. Original magnification 3100, inset, original magnifi- cation 3400. (B) IL-37 expression was quantified by immunoblotting and densitometric analysis (n = 6 per group). *p , 0.05 versus control. Equal amounts of proteins obtained from lung lysates were subjected to immunoblotting with anti–IL-37 Ab. The blots were stripped and reprobed with anti–b-actin Ab.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: IL-37 Attenuates Lung Fibrosis by Inducing Autophagy and Regulating TGF-β1 Production in Mice.

    doi: 10.4049/jimmunol.1801515

    Figure Lengend Snippet: FIGURE 2. IL-37 is expressed in the AECs and macrophages in the mouse lung and are decreased by BLM. (A) Immunohistochemical staining of IL-37 in mouse lungs. IL-37 was strongly localized in AECs (inset) and alveolar macrophages in the control lung. Original magnification 3100, inset, original magnifi- cation 3400. (B) IL-37 expression was quantified by immunoblotting and densitometric analysis (n = 6 per group). *p , 0.05 versus control. Equal amounts of proteins obtained from lung lysates were subjected to immunoblotting with anti–IL-37 Ab. The blots were stripped and reprobed with anti–b-actin Ab.

    Article Snippet: IL-37 knockdown in A549 cells Human lung cancer–derived AECs (A549 cells, ATCC CCL185; American Type Culture Collection, Manassas, VA) treated with or without BLM (10 mg/ml) were seeded on six-well plates and cultured in RPMI 1640 medium supplemented with 10% (v/v) FBS, 100 U/ml penicillin, and 100 mg/ml streptomycin.

    Techniques: Immunohistochemical staining, Staining, Control, Expressing, Western Blot

    FIGURE 3. Antiapoptotic effect of IL-37 on BLM-treated AECs. (A) Mouse primary AECs were exposed to BLM (10 mg/ml) and/or human IL-37 (10, 100, and 1000 ng/ml) for 24 h in serum-free medium. *p , 0.05 versus apoptosis or necrosis of the BLM+/IL-372–treated groups. Apoptosis was defined as Annexin V+/both PI+ and PI2. Necrosis was defined as PI+/both Annexin V+ and Annexin V2. (B) IL-37 siRNA and scrambled siRNA were transfected into A549 cells. BLM (10 mg/ml) was added 48 h post-siRNA transfection, and the samples were analyzed after 24 h. The cells were double stained with Annexin V/PI and analyzed by flow cytometry. *p , 0.05 versus apoptosis or necrosis of the scramble/BLM+-treated groups.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: IL-37 Attenuates Lung Fibrosis by Inducing Autophagy and Regulating TGF-β1 Production in Mice.

    doi: 10.4049/jimmunol.1801515

    Figure Lengend Snippet: FIGURE 3. Antiapoptotic effect of IL-37 on BLM-treated AECs. (A) Mouse primary AECs were exposed to BLM (10 mg/ml) and/or human IL-37 (10, 100, and 1000 ng/ml) for 24 h in serum-free medium. *p , 0.05 versus apoptosis or necrosis of the BLM+/IL-372–treated groups. Apoptosis was defined as Annexin V+/both PI+ and PI2. Necrosis was defined as PI+/both Annexin V+ and Annexin V2. (B) IL-37 siRNA and scrambled siRNA were transfected into A549 cells. BLM (10 mg/ml) was added 48 h post-siRNA transfection, and the samples were analyzed after 24 h. The cells were double stained with Annexin V/PI and analyzed by flow cytometry. *p , 0.05 versus apoptosis or necrosis of the scramble/BLM+-treated groups.

    Article Snippet: IL-37 knockdown in A549 cells Human lung cancer–derived AECs (A549 cells, ATCC CCL185; American Type Culture Collection, Manassas, VA) treated with or without BLM (10 mg/ml) were seeded on six-well plates and cultured in RPMI 1640 medium supplemented with 10% (v/v) FBS, 100 U/ml penicillin, and 100 mg/ml streptomycin.

    Techniques: Transfection, Staining, Cytometry